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Fermentation Atlas

Fermented milk from North Caucasus

Kefir (traditional grain kefir)кефир

Kefir is milk soured by kefir grains, small rubbery clusters of lactic bacteria, acetic bacteria and yeasts held together by protein and polysaccharide. The grains sit in milk at about 23–25 °C for about a day, are sieved out and reused. The drink is acid, slightly yeasty and lightly fizzy. Made in 5 steps over 24 h – 38 h. The longest step, ferment with occasional stirring, takes 12–24 h (FOODSAFE BC with the BC Ministry of Health 2022) and runs at 23–25 °C (Tetra Pak). Lactobacillus kefiranofaciens and Lentilactobacillus kefiri do most of the fermenting.

Key numbers
Main step12–24 hSet[bc-kefir][tp][walsh2016]Ferment with occasional stirring
Temperature23–25 °CReported[tp][walsh2016]Ferment with occasional stirring
AciditypH 4.4Reported[tp]Ripen cool, then chill

Recipe

Amounts from a sourceGrain-to-milk ratio from the Tetra Pak grain culture; households use no fixed batch size. BC food-service guidance uses 25 mL of commercial grains per 10 L of milk and fresh grains each time.
Milk (cow, goat or sheep) · Fat of 2.5–3.5 % is common; the milk is heated first[tp]
1 part to 20–30 parts milk (about 3.5–5 %)Kefir grains · One study used 2 % grains by weight in pasteurised whole milk[tp][walsh2016]
Starterkefir-grains

Total time across the recorded steps: 24 h – 38 h

Start
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90–95 °C

What changes: Heat kills stray bacteria and phages before the grains go in

Step 1 of 5
Heat the milk
Time
5 min
Reported
Temperature
90–95 °C
Reported
Salt
None given
Acidity
Not documented
Which microbes the steps name, step by step
Yeasts
Leuconostoc
Acetobacter rise
Flavour
Not documented
Texture
Not documented
Colour
Not documented

Microbes at work in this step

heat kills competing bacteria and phages that would harm the grains

Method

Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source

What makes this one different

Kefir is soured by a reusable grain that holds bacteria and yeasts together, so it is acid, a little alcoholic and fizzy. Yoghurt uses only two bacteria and no yeast. Koumiss is mare milk soured by a liquid starter, with no grains.

Related ferments

Variants

  • Industrial kefir (bulk starter)The grains make a mother culture, which starts a bulk starter at 2–3 %. The milk is homogenised at 65–70 °C and 175–200 bar, heated to 90–95 °C for 5 min, soured about 12 h at about 23 °C to pH 4.5, pre-cooled to 14–16 °C, ripened 12–14 h to pH about 4.4 and cooled to 5–8 °C.
  • Kefir from freeze-dried cultureStrains isolated from grains are sold as a concentrated culture, with no grains. Some countries do not allow the product to be called kefir and call it "kefir type".

Safety

Codex requires kefir to reach a titratable acidity of at least 0.6 % lactic acid and to hold at least 10^4 yeasts and 10^7 starter bacteria per gram. BC food-safety guidance uses pasteurised milk, sets pH 4.6 or lower as the critical limit, keeps kefir at 4 °C or colder and bans reusing grains between batches in food service.

History and sources

Every number is labelled with how it is known. How this atlas is made

Kefir comes from the Caucasus. Proteins in lumps found with Early Bronze Age burials at the Xiaohe cemetery in Xinjiang point to a kefir-like dairy there (Yang et al. 2014).

  1. [tp] Tetra Pak, Dairy Processing Handbook, chapter 13 Fermented milk products (kefir)book
  2. [cxs243] Codex Alimentarius, Standard for Fermented Milks CXS 243-2003 (revised 2008, 2010)spec
  3. [walsh2016] Walsh A.M. et al. 2016, Microbial succession and flavor production in the fermented dairy beverage kefir, mSystems 1(5):e00052-16paper
  4. [nejati2020] Nejati F., Junne S., Neubauer P. 2020, A big world in small grain, a review of natural milk kefir starters, Microorganisms 8(2):192paper
  5. [prado2015] Prado M.R. et al. 2015, Milk kefir, composition, microbial cultures, biological activities and related products, Frontiers in Microbiology 6:1177paper
  6. [bc-kefir] FOODSAFE BC with the BC Ministry of Health, BCCDC and Fraser Health, Guidelines for the Production of Dairy Products in Food Establishments, Kefir sample documentation, September 2022gov
  7. [yang2014] Yang Y., Shevchenko A. et al. 2014, Proteomics evidence for kefir dairy in Early Bronze Age China, Journal of Archaeological Science 45:178–186paper
  8. [wp] Wikipedia, Kefir (outline and history only)wiki