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Tempeh (tempe kedelai, traditional leaf-wrapped)tempe
Dehulled soybeans are soaked until they turn sour, boiled, cooled and mixed with spores of Rhizopus mould, then wrapped in leaves or perforated plastic. In one to two days near 30 °C the white mould grows through the beans and binds them into a firm cake that slices cleanly. Made in 5 steps over 2 days – 2.9 days. The longest step, incubate, takes 24–44 h (Tünçel G 1989) and runs at 20–30 °C (Tünçel G 1989). Rhizopus microsporus var. oligosporus and Rhizopus oryzae and R. stolonifer do most of the fermenting.
Amounts from a sourceTraditional makers have no fixed batch size. The water ratios and starter dose are from a Wageningen laboratory method for soybean tempe and starter cakes, built to match Indonesian practice; the Codex standard fixes what tempe may be made of.
Water · Once for the 24 h soak and again for boiling[tuncel1989]
0.3 % v/w spore suspension (laboratory dose)
Rhizopus starter · R. oligosporus, R. oryzae and/or R. stolonifer on rice flour, rice bran or wheat bran; traditionally usar, waru leaves carrying mould from an earlier batch[codex-tempe][tuncel1989]
No salt and no food additives · Codex allows only processing aids that control acidity during soaking[codex-tempe]
Starter
koji/qu/mold-starter: usar (Hibiscus tiliaceus 'waru' leaves carrying mould from a previous batch) or ragi tempe powder
Total time across the recorded steps: 2 days – 2.9 days
Start
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What changes:Lactic bacteria sour the soak water while the beans swell
Step 1 of 5
Soak until the beans sour
Time
24 h
Reported
Temperature
25 °C
Reported
Salt
None given
Acidity
pH 4–4.5
Measured
Which microbes the steps name, step by step
Lactic acid bacteria
Rhizopus spores
Rhizopus hyphae
Output: soured, soaked beans.
Flavour
slightly sour
Texture
swollen
Colour
Not documented
Microbes at work in this step
lactic acid bacteria sour the water; the acid holds back Enterobacteriaceae, B. cereus and S. aureus
Method
Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source
What makes this one different
Tempeh is a mould ferment like koji, but the mould is Rhizopus, not Aspergillus, and the whole cake is the food. Unlike natto the beans are soured first and the result is firm, not sticky. No salt is used.
Tempe murniplain soybean tempe in plastic, no additions
Tempe mondholunder-fermented; the mycelium does not yet cover the beans
Tempe semangitkept a few days past ripe until soft, yellowish and pungent; used as a seasoning
Tempe bosok (busuk)left until dark and strong-smelling; a little flavours sambal tumpang
Tempe mendoanthin sheets, battered and half-fried (Banyumas); a way of cooking, not a ferment
Safety
Souring the beans in the soak holds back Enterobacteriaceae, Bacillus cereus and Staphylococcus aureus; in a Dutch survey of retail tempe, about one sample in ten carried B. cereus or S. aureus at 10⁵ per g or more. Fresh tempe should smell clean, with no ammonia. Coconut-cake tempe (bongkrek) is a different and dangerous product.
Hazards the sources name: Bacillus cereus (10⁵ per g or more in 11 % of Dutch retail tempe); Staphylococcus aureus (10⁵ per g or more in 13 % of Dutch retail tempe); Enterobacteriaceae (10⁶ per g or more in 60 % of the same samples).
Shurtleff and Aoyagi trace the earliest known mention of tempe to the Javanese Serat Centhini. Gericke and Roorda's Javanese–Dutch dictionary listed it in 1875, Prinsen Geerligs first identified the mould in 1895, and Stahel wrote the first English article in 1946.