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Thua nao is northern Thailand's sticky, alkaline fermented soybean. Soaked soybeans are boiled for hours, packed into a bamboo basket lined with banana leaves and left warm for two to three days while Bacillus bacteria ferment them. The beans are mashed into a paste that is steamed or roasted in leaves, or pressed into thin discs and sun-dried to keep for months. Made in 5 steps over 2.1 days – 3.2 days. The longest step, ferment in a banana-leaf-lined bamboo basket, takes 2–3 days (Suriyanoi K 2018) and runs at 37–42 °C (Shurtleff W 1970). Bacillus, 82.7–86.8 % of bacteria in Shan thua nao; B. subtilis is the main species does most of the fermenting.
Salt, garlic, onion and red chilli · Mashed into the fermented beans for paste; the fermented beans themselves are unsalted[sic-natto][mathatheeranan2024]
Starter: spontaneous (bamboo basket and banana leaves); commercial Bacillus subtilis starters used in studies
Total time across the recorded steps: 2.1 days – 3.2 days
Start
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What changes:Soybeans are washed and soak overnight
Step 1 of 5
Wash and soak overnight
Time
Soaked overnight (Shan practice; Karen and Lawa skip soaking)
Reported
Temperature
Not documented
Salt
None given
Acidity
Not documented
Which microbes the steps name, step by step
Bacillus subtilis
Output: soaked beans.
Flavour
Not documented
Texture
swollen
Colour
Not documented
Microbes at work in this step
Not documented for this step.
Method
Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source
What makes this one different
Thua nao is a natto-type Bacillus ferment, but it is fermented in banana leaves in a bamboo basket rather than in rice straw, and most of it is mashed and cooked or dried into discs instead of eaten as whole sticky beans.
Thua nao kab (dried discs); Shan and Burmese pè bok byaPaste pressed into paper-thin 3-inch rounds and sun-dried; keeps about 6 months.
Thua nao powderDried discs ground to powder and stored for months.
Ferm-soy mix (1970)A nutrition-project mix of 60 % thua nao powder, 20 % fish meal and 6 % iodised salt, with chilli, garlic and onion powder.
Black soybean thua naoMade from black soybeans; drying method changes its aroma.
Safety
Market thua nao is often made without hygiene controls. E. coli was found in over half of market samples, with Salmonella and B. cereus in some, so fresh thua nao is steamed or roasted before eating. Fresh paste keeps only about two days; drying it into discs extends this to months.
Hazards the sources name: Escherichia coli in 54.17 % of 24 market samples; Salmonella spp. and Bacillus cereus also detected; Proteus in Shan thua nao.
Thua nao is the Shan name; Karen and Lawa make their own versions, tor-nor-au and kuem. Shurtleff and Aoyagi found no historical record of the product. In 1970 Sundhagul and colleagues developed a dried thua nao powder for a nutrition mix.