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The yellow juice pressed from grated bitter cassava is left to settle out its starch, then the liquid sours for about half a day to a day while lactic bacteria take over and part of the cyanide escapes. Boiling with garlic, herbs and salt drives off most of the rest and gives a sour, savoury yellow broth. Made in 6 steps over 51 days – 52 days. The longest step, cool, bottle and keep cold, takes up to 50 days (Campos A.P.R 2019) and runs at 10 °C (Campos A.P.R 2019). Lactobacillus fermentum and Weissella do most of the fermenting.
Amounts from a sourceThe seasoning amounts are the shares used in the Embrapa pilot process (Campos et al. 2019), given as percent of the fermented juice. Family workshops season by eye; one in Vigia de Nazaré makes about 1440 L of tucupi a month.
Manipueira · The juice pressed from grated yellow-fleshed bitter cassava; about 300 L comes from 1000 kg of roots[embrapa2013]
Total time across the recorded steps: 51 days – 52 days
Start
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What changes:Peeled bitter cassava roots soak in water before grating
Step 1 of 6
Peel, wash and soak the roots
Time
12 h
Reported
Temperature
Not documented
Salt
None given
Acidity
Not documented
Which microbes the steps name, step by step
Weissella
Lactobacillus fermentum
L. plantarum
Weissella dominant
Lactobacillus dominant
Output: soaked, peeled bitter cassava.
Flavour
Not documented
Texture
Not documented
Colour
Not documented
Microbes at work in this step
Not documented for this step.
Method
Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source
What makes this one different
Tucupi is made from the juice, not the pulp, of bitter cassava. It ferments for only about a day and then must be boiled, while gari and fufu ferment the grated or soaked root itself for days.
Tucupi powderSpray-dried with maltodextrin; the optimised trial used 214 °C inlet air and 27 % maltodextrin.
Starter-culture tucupiJuice inoculated with Pediococcus acidilactici kept Lactobacillus dominant and cut Bacillus and Enterococcus in a 24 h trial.
Safety
Bitter cassava juice is cyanogenic. Embrapa trials found that 24 h of fermentation and 10 min of boiling took total cyanide below 10 mg HCN/L, but 22 market samples in Belém held 8.87–114.66 mg/L total HCN and up to 66.8 mg/L histamine, so short boiling is a real risk. Pará's standard (ADEPARÁ Normative Instruction 1/2008) sets pH 3.5–4.3 and 0.1–0.8 g lactic acid per 100 mL, and requires the absence of Salmonella, coagulase-positive staphylococci and Bacillus cereus.
Hazards the sources name: Bacillus cereus, Salmonella and coagulase-positive Staphylococcus (the Pará standard requires their absence; none were found in the Embrapa product).
Tucupi is a staple of Pará cooking and a by-product of farinha making; families and small agroindustries in Pará bottle it for markets such as Ver-o-Peso, and demand roughly doubles prices during the Círio de Nazaré in October.
[brito2019] do Carmo Brito B.N., Chisté R.C., Lopes A.S., Gloria M.B.A., Pena R.S. 2019, Influence of spontaneous fermentation of manipueira on bioactive amine and carotenoid profiles during tucupi production, Food Research International 120:209–216, doi:10.1016/j.foodres.2019.02.040 (abstract)paper