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Fermentation Atlas

Soy and legumes from Japan — Nagano

Kome miso, salty light type (Shinshu miso) — reference process米味噌

Steamed rice is grown into koji, then mixed with crushed cooked soybeans, salt and water and left in tanks while koji enzymes, salt-tolerant yeast and lactic bacteria work on it. Shinshu miso is the salty, light-yellow type, ripened for only 2–3 months. Rice miso is about 80 % of the miso Japan makes, and Nagano makes the largest share of it. Made in 6 steps over 2.1 months – 3.1 months. The longest step, ferment and ripen, takes 2–3 months (Itō M) and runs at 25–30 °C (Kusumoto K-I 2021). Salt is 11–13 % (Itō M). Aspergillus oryzae and Zygosaccharomyces rouxii do most of the fermenting.

Key numbers
Main step2–3 monthsSet[aichi-itc]Ferment and ripen
Temperature25–30 °CReported[kusumoto2021]Ferment and ripen
Salt11–13 %Set[aichi-itc][kitagawa2019]Crush the beans and mix with koji, salt and water (shikomi)
AciditypH 5Reported[kitagawa2019][kusumoto2021]Ferment and ripen
Fully documentedSoy and legumes2.1 months – 3.1 monthsMore soy and legumesMore from Japan

Recipe

Amounts from a sourceHome recipe from a Kyoto Prefecture agricultural extension sheet, ripened 6–12 months in a weighted tub. Factory Shinshu miso uses the same three ingredients with less koji (5–10 bu) and warm tanks, so it is ready in 2–3 months.
1.3 kg (1 shō)Soybeans · Soaked 12–15 h in 2.5 times as much water, then boiled in the soaking water[kyoto-tango]
1.65 kgRice koji · Made from 1 shō of rice[kyoto-tango]
660 gSalt · Two handfuls kept back to scatter on the surface; gives about 11 % salt in the miso[kyoto-tango]
As neededBean cooking water · Added only if the mix is crumbly[kyoto-tango]
Starterkoji/qu/mold-starter (tane-koji: Aspergillus oryzae); salt-tolerant yeast and lactic bacteria added at packing

Total time across the recorded steps: 2.1 months – 3.1 months

Start
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What changes: Steam cooks the rice through so the koji mould can grow into each grain

Step 1 of 6
Wash, soak and steam the rice
Time
40 min – 1 h
Reported
Temperature
Not documented
Salt
None given
Acidity
Not documented
Which microbes the steps name, step by step
Moulds
Lactic acid bacteria
Yeasts

Steaming must gelatinise the starch fully, or koji hyphae cannot grow into the grain. Output: steamed rice.

Flavour
Not documented
Texture
firm grains with fully gelatinised starch
Colour
Not documented

Microbes at work in this step

steam kills what is on the grain

Method

Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source

What makes this one different

Shinshu miso uses little koji for a rice miso. The koji rice weighs 0.5–1.0 times the soybeans, against 1.5–3 times in sweet white miso, and the salt is 11–13 %. A short, warm ripening keeps it pale. Sendai miso has the same koji ratio and salt but ripens 3–12 months and turns red.

Related ferments

Safety

Salty miso has about 12 % salt, which keeps unwanted microbes down during long ripening. Miso and koji are not acid foods, so pH is not their safety hurdle; clean raw materials and hygiene matter most. Koji strains used in Japan do not make aflatoxin.

History and sources

Every number is labelled with how it is known. How this atlas is made

Shurtleff and Aoyagi record that the warlord Takeda Shingen had miso made in Shinshu to feed his troops. Today Nagano Prefecture ships more miso than any other prefecture.

  1. [kitagawa2019] Kitagawa M. 2019, 発酵調味料「味噌」を知る (Getting to know miso, a fermented seasoning), Seibutsu-kogaku Kaishi 97(4):207–209, Society for Biotechnology, Japanpaper
  2. [aichi-itc] Itō M., 日本の伝統的発酵調味料である味噌について (On miso), Aichi Center for Industry and Science Technology, Food News No. 16, p. 3; type table adapted from みそ技術ハンドブックgov
  3. [kusumoto2021] Kusumoto K-I., Yamagata Y., Tazawa R., Kitagawa M., Kato T., Isobe K., Kashiwagi Y. 2021, Japanese traditional miso and koji making, Journal of Fungi 7(7):579, doi:10.3390/jof7070579paper
  4. [allwood2023] Allwood J.G., Wakeling L.T., Bean D.C. 2023, Microbial ecology of Australian commercial rice koji and soybean miso, JSFA Reports 3:207–221, doi:10.1002/jsf2.112paper
  5. [kyoto-tango] Kyoto Prefecture, Tango agricultural extension, みそ (home miso recipe sheet)gov
  6. [sic-miso2] Shurtleff W., Aoyagi A., History of Miso and Soybean Jiang, SoyInfo Center (chapter 2)book
  7. [JA-miso] Japanese Wikipedia, 味噌 (miso; type table of koji ratio, salt and ageing)wiki