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In the traditional Chinese sour-liquid method, mung beans are soaked, wet-milled and sieved, and sour liquid from an earlier batch is stirred into the starch milk. Its lactic bacteria acidify the milk to about pH 4 and make the starch flocculate and settle fast. The clean starch is dried and later made into clear glass noodles by boiling, freezing and drying. Made in 6 steps over 19 h – 2.2 days. The longest step, freeze, then dry, takes 19 h – 2.2 days (Photinam R 2025) and runs at -10 °C (Photinam R 2025). Leuconostoc mesenteroides, Lacticaseibacillus casei, Lactobacillus cellobiosus and Limosilactobacillus fermentum in the steep water and Lactococcus (37.6–54.5 % of bacteria), Streptococcus and Lactobacillus in Beijing douzhir, the sour liquid left after starch making; Lactococcus lactis isolates flocculate starch do most of the fermenting.
Amounts from a sourceNoodle amounts are a 1 kg laboratory starch blend from a 2025 Thai study. The starch fermentation is a workshop process with no fixed amounts in the sources.
Sour steep water from a previous fermentation · Water that has fermented for 12 h, used as the inoculum[fao1999]
950 g + 50 g
Raw mung bean starch plus pre-gelatinised starch · For the noodle dough; the 50 g is cooked with 250 ml water for 5 min as a binder[photinam2025]
Starter
backslop (sour liquid from the previous batch)
Total time across the recorded steps: 19 h – 2.2 days
Start
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What changes:Mung beans soak in sour steep water saved from the last batch
Step 1 of 6
Soak the mung beans with old sour liquid
Time
Not documented
Temperature
Not documented
Salt
None given
Acidity
pH 4
Reported
Which microbes the steps name, step by step
Lactic acid bacteria
Output: soaked, softened beans.
Flavour
Not documented
Texture
Not documented
Colour
Not documented
Microbes at work in this step
lactic acid bacteria from the old steep water acidify the soak
Method
Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source
What makes this one different
The ferment here is a tool, not the food. Lactic bacteria in the sour liquid separate and protect the starch; the noodles themselves are boiled and dried and are not sour.
Centrifuge-separated starchStarch spun out by centrifuge instead of settled with sour liquid; widely used in China, with lower yield and less clear noodles.
Beijing douzhirThe sour, grey-green liquid left on top, drunk as a fermented drink.
Luoyang mung bean sour liquidMung beans soaked about 12 h, ground with 15–20 times water, sieved and left to sour, then used as the broth for sour-liquid noodles.
Safety
The acid sour liquid (about pH 4) keeps the starch from spoiling while it settles. Uncooked douzhir samples from Beijing makers carried Klebsiella as well as lactic bacteria.
Hazards the sources name: Klebsiella (4.9–24.2 % of bacteria in douzhir samples).
Beijing douzhir, the sour liquid left after starch making, has been drunk for at least 1000 years. Starch from sour-liquid processing is still prized for noodles in China.
[liu2007] Liu W., Shen Q. 2007, Studies on the physicochemical properties of mung bean starch from sour liquid processing and centrifugation, Journal of Food Engineering 79(1):358–363 (abstract)paper