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Fermentation Atlas

Sauces and condiments from Japan

Koikuchi shoyu (Japanese dark soy sauce, honjōzō brewed) — reference process濃口醤油

Koikuchi is Japan's everyday dark soy sauce. Steamed soybeans and roasted, cracked wheat in about equal amounts are grown into koji, mixed with brine and fermented for about 6–8 months, then pressed and heated. It is over 80 % of the soy sauce made in Japan. Made in 6 steps over 6.1 months – 12 months. The longest step, ferment and age the moromi, takes 6–12 months (MAFF) and runs at 15–28 °C (Luh B.S. 1995). Salt is 17–19 % (Luh B.S. 1995). Aspergillus oryzae / A. sojae and Tetragenococcus halophilus do most of the fermenting.

Key numbers
Main step6–12 monthsReported[maff-shoyu][maff-hakko][luh1995]Ferment and age the moromi
Temperature15–28 °CReported[luh1995]Ferment and age the moromi
Salt17–19 %Reported[luh1995]Mix the koji with brine (moromi)
AciditypH 4.7Reported[luh1995]Heat (hi-ire) and bottle
Fully documentedSauces and condiments6.1 months – 12 monthsMore sauces and condimentsMore from Japan

Recipe

Amounts from a sourceProportions from Luh's 1995 flow sheet for industrial soy sauce. The Japanese Agricultural Standard defines koikuchi by its koji, made from soybeans and about the same amount of wheat.
50 partsSoybeans or defatted soybean flakes · Soaked, then steamed under pressure[luh1995]
50 partsWheat · Roasted and cracked[luh1995][jas-shoyu]
0.1–0.2 %Tane-koji (Aspergillus oryzae or A. sojae spores)[luh1995]
200 partsBrine, 20 % salt · A government guide gives 1–1.2 times the koji volume[luh1995][maff-hakko]
Starterkoji/qu/mold-starter (Aspergillus oryzae or A. sojae tane-koji); salt-tolerant lactic bacteria and yeasts in the brine

Total time across the recorded steps: 6.1 months – 12 months

Start
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170 °C

What changes: Steam softens the soybeans while hot air roasts the wheat

Step 1 of 6
Steam the soybeans; roast and crack the wheat
Time
0.3–45 min
Reported
Temperature
170 °C
Reported
Salt
None given
Acidity
Not documented
Which microbes the steps name, step by step
Moulds
Lactic acid bacteria
Yeasts

Output: cooked soybeans and cracked roasted wheat.

Flavour
Not documented
Texture
Not documented
Colour
Not documented

Microbes at work in this step

heat kills the raw-material flora

Method

Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source

What makes this one different

Koikuchi koji uses about equal soybeans and wheat. Tamari koji is nearly all soybean and shiro shoyu is mostly wheat. Usukuchi uses the same koji but more salt and steps that keep the colour pale. Only brewed (honjōzō) koikuchi can carry the JAS special grade.

Related ferments

Variants

  • Koikuchi made by mixed brewing (kongō jōzō) or mixing (kongō)amino-acid liquid from acid-hydrolysed protein added to the moromi or to the sauce; may be JAS upper or standard grade, never special grade

Made the koikuchi shoyu way

Safety

With about 17 % salt, brewed soy sauce keeps at room temperature. Naturally brewed soy sauce has little or no 3-MCPD; that contaminant comes from acid-hydrolysed vegetable protein added to some cheaper sauces.

History and sources

Every number is labelled with how it is known. How this atlas is made

Shurtleff and Aoyagi date the first Japanese mention of soy sauce to AD 775 and the word shoyu to 1597; John Locke's 'saio' of 1679 is the first English reference.

  1. [luh1995] Luh B.S. 1995, Industrial production of soy sauce, Journal of Industrial Microbiology 14:467–471, doi:10.1007/BF01573959paper
  2. [jas-shoyu] MAFF, Japanese Agricultural Standard for soy sauce (しょうゆの日本農林規格), MAFF Notification No. 1703 of 13 September 2004spec
  3. [maff-hakko] MAFF 2023, にっぽんの発酵食品 (Japan's fermented foods), chapter 3, soy sauce tablegov
  4. [maff-shoyu] MAFF, しょうゆの製造方法 (how soy sauce is made; honjōzō share and moromi time)gov
  5. [cfs2019] Centre for Food Safety, Hong Kong 2019, The process contaminant 3-MCPD in condiments, Food Safety Focus 156gov
  6. [sic-shoyu] Shurtleff W., Aoyagi A., History of Soy Sauce, Shoyu and Tamari, SoyInfo Centerbook