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Fermentation Atlas

Starters and koji from Ethiopia, Eritrea

Ersho (injera starter liquid)እርሾ

Ersho is the clear yellow liquid that collects on top of fermenting teff batter. Households save some of it and stir it into the next batch of flour and water, so each injera batter is started from the last one. It is very sour, about pH 3.5 to 4, and full of yeasts and bacteria. Made in 3 steps over 24 h – 3 days. The longest step, ferment until a yellow liquid rises, takes 24 h – 3 days (Hagere Hailemariam 2024) and runs at 25 °C (Hagere Hailemariam 2024). Candida milleri and Other yeasts Rhodotorula mucilaginosa, Kluyveromyces marxianus, Pichia naganishii and Debaryomyces hansenii do most of the fermenting.

Key numbers
Main step24 h – 3 daysReported[hagere2024]Ferment until a yellow liquid rises
Temperature25 °CMeasured[hagere2024]Ferment until a yellow liquid rises
AciditypH 3.5–4Measured[ashenafi1994][hagere2024]Pour off the liquid and save some as ersho
Fully documentedStarters and koji24 h – 3 daysMore starters and kojiMore from Ethiopia

Recipe

No fixed amounts recordedHouseholds use a small, unmeasured portion of ersho; no source gives a standard amount.
Ersho saved from the last batter · A small portion starts the next batch[ashenafi1994][hagere2024]
Teff flour and water for the next batter[hagere2024]
Starter: backslop

Total time across the recorded steps: 24 h – 3 days

Start
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What changes: Saved ersho is stirred into teff flour and water

Step 1 of 3
Mix teff flour, water and saved ersho into a thin batter
Time
Not documented
Temperature
Not documented
Salt
None given
Acidity
Not documented
Which microbes the steps name, step by step
Yeasts
Lactic acid bacteria
Flavour
Not documented
Texture
Not documented
Colour
Not documented

Microbes at work in this step

Not documented for this step.

Method

Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source

What makes this one different

Ersho is a liquid starter, not a dough or a dried cake. It is skimmed off the top of the old batter rather than kept as a lump of dough, as most sourdough starters are.

Related ferments

Variants

  • Defined-consortium ersho (2024 thesis)Lactic bacteria, Bacillus, yeast and mould isolates from 91 ersho samples were combined into one starter; the best-liked injera came from a 28-hour fermentation.

Safety

Ersho is very acidic, around pH 3.5 to 4, which holds back most unwanted bacteria. Teff flour itself carried Enterobacteriaceae at 10⁴ cfu/g in about 80 % of samples in one study, so the acid from a strong ersho matters.

History and sources

Every number is labelled with how it is known. How this atlas is made

Ashenafi's 1994 study of four households' ersho named Candida milleri as its dominant yeast. A 2024 thesis collected 91 samples to build a defined starter from it.

  1. [ashenafi1994] Ashenafi M. 1994, Microbial flora and some chemical properties of ersho, a starter for teff (Eragrostis tef) fermentation, World Journal of Microbiology and Biotechnology 10(1):69–73, doi:10.1007/BF00357567 (abstract)paper
  2. [hagere2024] Hagere Hailemariam 2024, Evaluation of microbes from teff (Eragrostis tef) ersho for injera starter culture development, MSc thesis, Institute of Biotechnology, Addis Ababa Universitypaper
  3. [mengesha2022] Mengesha Y., Tebeje A., Tilahun B. 2022, A review on factors influencing the fermentation process of teff (Eragrostis teff) and other cereal-based Ethiopian injera, Int. J. Food Sci. 2022:4419955paper