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Soft drinks from Uganda

Obushera (obutoko type)obushera

Obutoko is the basic Ugandan obushera, a sweet-sour drink of malted sorghum. Malt flour is mixed with water, heated and cooled, then a little raw malt is stirred in to start a spontaneous lactic and yeast fermentation that sours it within one to three days. Adding honey and fermenting further turns it into the alcoholic enturire.

Fully documentedSoft drinks5 days

Recipe

Amounts from a sourceA lab batch made by the traditional spontaneous method (Mukisa et al. 2010, as described by Mukisa, Ntaate and Byakika 2017); household amounts are not fixed.
Sorghum malt flour125 gMixed into 1 L of water to make the slurry[mukisa2017e]
Water1 L[mukisa2017e]
Raw sorghum malt22.5 gStirred into the cooled slurry to start the fermentation[mukisa2017e]
Starterspontaneous (raw malt supplies the flora); defined starters have been tested

Total time across the recorded steps: 5 days

Start
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shapes, not to countLactobacillusLb. fermentumLb. delbrueckiiLactococcus lactis60 °C

What changes: Soaked sorghum sprouts on trays and is dried into malt

Step 1 of 4
Malt the sorghum
Time
2 days
Reported
Temperature
60 °C
Reported
Salt
None given
Acidity
Not documented
Which microbes the steps name, step by step
Lactobacillus
Lb. fermentum
Lb. delbrueckii
Lactococcus lactis
Lactic acid bacteria

Output: sorghum malt, ground to flour.

Flavour
Not documented
Texture
Not documented
Colour
Not documented

Microbes at work in this step

Not documented for this step.

Method

Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source

At a glance

Obutoko is the basic Ugandan obushera, a sweet-sour drink of malted sorghum. Malt flour is mixed with water, heated and cooled, then a little raw malt is stirred in to start a spontaneous lactic and yeast fermentation that sours it within one to three days. Adding honey and fermenting further turns it into the alcoholic enturire. Made in 4 steps over 5 days. The longest step, ferment, takes 3 days (Mukisa I.M 2017) and runs at 25 °C (Mukisa I.M 2017). Lactic acid bacteria isolated from obushera: Lactobacillus (now Lactiplantibacillus) plantarum, Lb. fermentum, Lb. delbrueckii, Lactococcus lactis, Leuconostoc lactis, Pediococcus pentosaceus and Weissella confusa and Saccharomyces cerevisiae (the predominant yeast in obutoko by DGGE); Pichia spp. and Issatchenkia orientalis also detected do most of the fermenting.

Key numbers
Main step3 daysReported[mukisa2017e]Ferment
Temperature25 °CReported[mukisa2017e]Ferment
AciditypH 4.6–6 → 3.5Measured[mukisa2012]Ferment

What makes this one different

Obutoko is made only from sorghum malt, so the malt both sweetens the slurry and brings the microbes. Ekitiribita sours more slowly (pH 4.4 after 4 days, against 3.5–4.0 within 1–2 days for obutoko), and enturire is obutoko fermented again with honey.

Related ferments

Variants

  • EnturireObutoko sweetened with honey and fermented 2–4 days more; alcoholic (see `enturire`).

Safety

In a spontaneous lab fermentation the pH took 24 h to fall to 3.55–3.83, against 12 h with lactic starters. Acid-tolerant E. coli, some with Shiga-toxin genes, have been isolated from commercial obushera, and most processors do not pasteurise the drink. In a Mbarara survey, sold obushera carried staphylococci, Klebsiella and other contaminants, processors' handling and hygiene did not conform to good manufacturing practice, and counts did not meet the East African standard. A 2003 survey records families giving it to babies from 5–7 months. That is a record of custom, not advice. The lab malt method soaks the grain in 0.2 % sodium hydroxide (lye) and then rinses it. Lye is corrosive. It burns skin and eyes, and swallowing it can severely injure the mouth, gullet and stomach [atsdr-naoh]. US rules accept it as a food ingredient only when it meets the Food Chemicals Codex, so use food-grade lye, never drain cleaner [cfr-naoh].

Hazards the sources name: Coliforms (across the four obushera types, 2.0 log cfu/g falling to below 1 log cfu/g during four days of fermentation); Acid-tolerant Escherichia coli, some carrying the Stx2 gene, isolated from commercial obushera; Coagulase-positive staphylococci, Klebsiella, Enterobacter and other contaminants in obushera sold in Mbarara.

History and sources

Every number is labelled with how it is known. How this atlas is made

Muyanja et al. (2003) surveyed 48 households in Kabale and 50 in Rukungiri and called bushera the most common traditional fermented beverage of south-western Uganda, made at household level by spontaneous fermentation. They noted that its fermenting microbes had not yet been isolated and characterised. Mukisa et al. (2012) later identified them by sequencing.

  1. [muyanja2003] Muyanja C.M.B.K., Kikafunda J.K., Narvhus J.A., Helgetun K., Langsrud T. 2003, Production methods and composition of bushera, a Ugandan traditional fermented cereal beverage, African Journal of Food, Agriculture, Nutrition and Development 3(1)paper
  2. [mukisa2012] Mukisa I.M., Porcellato D., Byaruhanga Y.B., Muyanja C.M., Rudi K., Langsrud T., Narvhus J.A. 2012, The dominant microbial community associated with fermentation of Obushera (sorghum and millet beverages) determined by culture-dependent and culture-independent methods, International Journal of Food Microbiology 160:1–10 (abstract)paper
  3. [mukisa2017f] Mukisa I.M., Byaruhanga Y.B., Muyanja C.M.B.K., Langsrud T., Narvhus J.A. 2017, Production of organic flavor compounds by dominant lactic acid bacteria and yeasts from Obushera, a traditional sorghum malt fermented beverage, Food Science & Nutrition 5:702–712paper
  4. [mukisa2017e] Mukisa I.M., Ntaate D., Byakika S. 2017, Application of starter cultures in the production of Enturire, a traditional sorghum-based alcoholic beverage, Food Science & Nutrition 5:609–616paper
  5. [byakika2019] Byakika S., Mukisa I.M., Mugabi R., Muyanja C. 2019, Antimicrobial activity of lactic acid bacteria starters against acid tolerant, antibiotic resistant, and potentially virulent E. coli isolated from a fermented sorghum-millet beverage, International Journal of Microbiology 2019:2013539paper
  6. [atsdr-naoh] Agency for Toxic Substances and Disease Registry (CDC), Medical Management Guidelines for Sodium Hydroxidegov
  7. [cfr-naoh] US Code of Federal Regulations, 21 CFR 184.1763, Sodium hydroxidegov
  8. [kiprotich2022] Kiprotich A., Wangalwa R., Atwebembeire J., Kagoro-Rugunda G. 2022, Microbial contamination and antimicrobial susceptibility pattern of bacterial isolates from sorghum and millet derived local beverage (Obushera) consumed in Mbarara City, Uganda, preprint, not peer reviewed (abstract, via Europe PMC)other