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Vinegar from Worldwide industrial practice, from the 20th century

Submerged fermentation vinegar (acetator)

Most industrial vinegar today is made in a stirred stainless-steel tank, the acetator, where a turbine blows very fine air bubbles through wine, cider, beer or dilute spirit. Acetic bacteria float freely in the liquid and turn ethanol into acetic acid in about a day per cycle. Part of each batch is drawn off and the rest seeds the next fresh load.

Fully documentedVinegar24 h – 27 hFamily: Orléans method (slow surface vinegar)

Recipe

No fixed amounts recordedSemi-continuous cycles in an 8 L pilot Frings acetator (Román-Camacho et al. 2022); the review gives industrial acetator volumes of 20,000–100,000 L [trends2023].
Alcoholic medium (wine, beer, fruit wine or dilute spirit)Loaded so that ethanol does not exceed a preset level, 5 % (v/v) in one pilot plant[trends2023]
Vinegar left in the tank from the previous cycleThe unloaded half is replaced and the rest acts as the inoculum[trends2023][rc2022]
Startervinegar left from the previous cycle, used as the inoculum of the next

Total time across the recorded steps: 24 h – 27 h

Start
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What changes: Wine or beer is thinned with water to about 10 percent alcohol before it goes in

Step 1 of 4
Dilute the wine or beer with water to the working strength
Time
Not documented
Temperature
Not documented
Salt
None given
Acidity
Not documented
Which microbes the steps name, step by step
K. europaeus
Komagataeibacter
Gluconacetobacter

Output: medium at about 10 % ethanol.

Flavour
Not documented
Texture
Not documented
Colour
Not documented

Microbes at work in this step

Not documented for this step.

Method

Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source

At a glance

Most industrial vinegar today is made in a stirred stainless-steel tank, the acetator, where a turbine blows very fine air bubbles through wine, cider, beer or dilute spirit. Acetic bacteria float freely in the liquid and turn ethanol into acetic acid in about a day per cycle. Part of each batch is drawn off and the rest seeds the next fresh load. Made in 4 steps over 24 h – 27 h. The longest step, acetify with fine air bubbles at constant temperature, takes 21–24 h (Román-Camacho J.J 2022) and runs at 30–31 °C (Román-Camacho J.J 2023). Komagataeibacter europaeus and Other Komagataeibacter and former Gluconacetobacter species, tolerant of 15–20 % acetic acid do most of the fermenting.

Key numbers
Main step21–24 hMeasured[rc2022]Acetify with fine air bubbles at constant temperature
Temperature30–31 °CReported[trends2023][rc2022]Acetify with fine air bubbles at constant temperature

What makes this one different

Instead of a mother film on still wine (Orléans) or bacteria fixed on wood shavings (quick process), the bacteria float in the liquid and are fed air as fine bubbles, so a cycle takes about a day [rc2022, trends2023]. The product is high in acetic acid but makes few other metabolites and loses volatile aromas [mas2014].

Related ferments

Variants

  • Batch and continuous modesBatch runs need a fresh starter each time; continuous runs top out at about 8–10 % (w/v) acidity [trends2023].
  • Two-stage high-strength processTwo tanks run in step to reach acetic acid above 20 % (w/v) [trends2023].

Safety

In the 8 L pilot acetator, cycles ended at 7.9 % (w/v) acetic acid for wine and 6.8 % for beer [rc2022].

History and sources

Every number is labelled with how it is known. How this atlas is made

In the 20th century the invention of submerged fermentation cut vinegar production times to 1-2 days and allowed cheap vinegar to be made around the world [WP:Vinegar]. Early containers for submerged culture were made of wood; most current ones are stainless steel [mas2014].

  1. [trends2023] Román-Camacho J.J., García-García I., Santos-Dueñas I.M., García-Martínez T., Mauricio J.C. 2023, Latest trends in industrial vinegar production and the role of acetic acid bacteria - a comprehensive review, Foods 12(19):3705, doi:10.3390/foods12193705paper
  2. [rc2022] Román-Camacho J.J., Mauricio J.C., Santos-Dueñas I.M., García-Martínez T., García-García I. 2022, Unraveling the role of acetic acid bacteria comparing two acetification profiles from natural raw materials - a quantitative approach in Komagataeibacter europaeus, Frontiers in Microbiology 13:840119, doi:10.3389/fmicb.2022.840119paper
  3. [mas2014] Mas A., Torija M.J., García-Parrilla M.C., Troncoso A.M. 2014, Acetic acid bacteria and the production and quality of wine vinegar, The Scientific World Journal 2014:394671, doi:10.1155/2014/394671paper
  4. [WP:Vinegar] Wikipedia, Vinegar (outline only)wiki