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Roots and tubers from Brazil

Polvilho azedo / almidón agrio (sour cassava starch)polvilho azedo

Wet cassava starch is left under a layer of water in tanks for weeks while lactic bacteria sour it, then spread thin and dried in the sun. Fermentation and sunlight together give the starch its power to puff in the oven without gluten or yeast, for pão de queijo and biscoito de polvilho.

Fully documentedRoots and tubers20 days – 2.3 months

Recipe

No fixed amounts recordedFactory or farm process with no fixed amounts; starch and water are the whole recipe. One factory tank held 16,000 L (Penido 2018).
Cassava rootsThe starch is extracted from the roots; sweet and sour starch go through the same extraction[penido2018]
WaterKept over the starch as a layer during fermentation[oliveira2021]
Starterspontaneous (microbes from the raw material and the tanks)

Total time across the recorded steps: 20 days – 2.3 months

Start
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What changes: Starch is separated out of the cassava roots before it is fermented

Step 1 of 4
Extract the starch from the cassava roots
Time
Not documented
Temperature
Not documented
Salt
None given
Acidity
Not documented
Which microbes the steps name, step by step
Lactic acid bacteria
Moulds

Output: wet native starch.

Flavour
Not documented
Texture
Not documented
Colour
Not documented

Microbes at work in this step

Not documented for this step.

Method

Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source

At a glance

Wet cassava starch is left under a layer of water in tanks for weeks while lactic bacteria sour it, then spread thin and dried in the sun. Fermentation and sunlight together give the starch its power to puff in the oven without gluten or yeast, for pão de queijo and biscoito de polvilho. Made in 4 steps over 20 days – 2.3 months. The longest step, ferment under a layer of water, takes 20 days – 2.3 months (Penido F.C.L 2018) and runs at 22 °C (Oliveira D.I 2021). Lactiplantibacillus plantarum and Limosilactobacillus fermentum, 6.0–9.0 log cfu/g in factory sour starch and Leuconostoc citreum, 47.6 % of clones on day 5 do most of the fermenting.

Key numbers
Main step20 days – 2.3 monthsReported[penido2018][lacerda2011][oliveira2021]Ferment under a layer of water
Temperature22 °CMeasured[oliveira2021]Ferment under a layer of water
AciditypH 4 → 3.46Measured[penido2018]Ferment under a layer of water

What makes this one different

Sweet starch (polvilho doce) is the same starch fermented only 2–7 days to about 1 % acidity; sour starch ferments 20–70 days to about 5 % and is sun-dried, which gives it its oven expansion.

1978 Brazilian acidity limit
Titratable acidity of 5.00 ml NaOH N/100 g at most; 52 % of 52 commercial samples were over it with no loss of quality
1978 Brazilian moisture limit
14 % at most; 58 % of samples were over it
Organic acids in commercial sour starch
Lactic 0.42 g/100 g, butyric 0.06 g/100 g, acetic 0.02 g/100 g; water activity 0.57 on average
Oven expansion
3.0–8.6 ml/g across 13 Colombian genotypes after 30 days of fermentation and sun drying

Related ferments

Variants

  • Polvilho doceSweet starch, fermented 2–7 days; does not expand the same way.
  • Almidón agrio (Colombia)Colombian sour starch; fermented 30 days and sun-dried in the Alvarado study of 13 genotypes.

Safety

Bacillus cereus was found at low numbers in factory sour starch. Commercial samples averaged a water activity of 0.57, too dry for most microbes to grow in storage.

Hazards the sources name: Bacillus cereus, found at low numbers in product from both factories studied.

History and sources

Every number is labelled with how it is known. How this atlas is made

Maeda (1999) proposed a national grading of sour starch into types A, B and C by oven expansion, as the 1978 limits did not fit commercial product.

  1. [oliveira2021] Oliveira D.I., Demogalski L., Dias A.H., Pereira L.A.A., Alberti A., Los P.R., Demiate I.M. 2021, Traditional sour cassava starch obtained with alterations in the solar drying stage, Food Science and Technology (Campinas) 41 suppl 1, doi:10.1590/fst.16120paper
  2. [penido2018] Penido F.C.L., Piló F.B., Sandes S.H.C. et al. 2018, Selection of starter cultures for the production of sour cassava starch in a pilot-scale fermentation process, Brazilian Journal of Microbiology 49:823–831paper
  3. [maeda1999] Maeda K.C. 1999, Proposta de classificação para o polvilho azedo, MSc dissertation, ESALQ, Universidade de São Paulo, doi:10.11606/D.11.1999.tde-20220207-231043 (abstract)paper
  4. [lacerda2005] Lacerda I.C.A., Miranda R.L., Borelli B.M. et al. 2005, Lactic acid bacteria and yeasts associated with spontaneous fermentations during the production of sour cassava starch in Brazil, International Journal of Food Microbiology 105:213–219 (abstract)paper
  5. [lacerda2011] Lacerda I.C., Gomes F.C., Borelli B.M. et al. 2011, Identification of the bacterial community responsible for traditional fermentation during sour cassava starch, cachaça and Minas cheese production, Brazilian Journal of Microbiology 42:650–657paper
  6. [alvarado2013] Alvarado P.M., Grosmaire L., Dufour D. et al. 2013, Combined effect of fermentation, sun-drying and genotype on breadmaking ability of sour cassava starch, Carbohydrate Polymers 98:1137–1146 (abstract)paper
  7. [ampe2001] Ampe F., Sirvent A., Zakhia N. 2001, Dynamics of the microbial community responsible for traditional sour cassava starch fermentation, International Journal of Food Microbiology 65:45–54 (abstract)paper