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Wet cassava starch is left under a layer of water in tanks for weeks while lactic bacteria sour it, then spread thin and dried in the sun. Fermentation and sunlight together give the starch its power to puff in the oven without gluten or yeast, for pão de queijo and biscoito de polvilho.
Fully documentedRoots and tubers20 days – 2.3 months
Recipe
No fixed amounts recordedFactory or farm process with no fixed amounts; starch and water are the whole recipe. One factory tank held 16,000 L (Penido 2018).
Cassava roots
The starch is extracted from the roots; sweet and sour starch go through the same extraction[penido2018]
Water
Kept over the starch as a layer during fermentation[oliveira2021]
Starter
spontaneous (microbes from the raw material and the tanks)
Total time across the recorded steps: 20 days – 2.3 months
Start
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What changes:Starch is separated out of the cassava roots before it is fermented
Step 1 of 4
Extract the starch from the cassava roots
Time
Not documented
Temperature
Not documented
Salt
None given
Acidity
Not documented
Which microbes the steps name, step by step
Lactic acid bacteria
Moulds
Output: wet native starch.
Flavour
Not documented
Texture
Not documented
Colour
Not documented
Microbes at work in this step
Not documented for this step.
Method
Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source
At a glance
Wet cassava starch is left under a layer of water in tanks for weeks while lactic bacteria sour it, then spread thin and dried in the sun. Fermentation and sunlight together give the starch its power to puff in the oven without gluten or yeast, for pão de queijo and biscoito de polvilho. Made in 4 steps over 20 days – 2.3 months. The longest step, ferment under a layer of water, takes 20 days – 2.3 months (Penido F.C.L 2018) and runs at 22 °C (Oliveira D.I 2021). Lactiplantibacillus plantarum and Limosilactobacillus fermentum, 6.0–9.0 log cfu/g in factory sour starch and Leuconostoc citreum, 47.6 % of clones on day 5 do most of the fermenting.
Sweet starch (polvilho doce) is the same starch fermented only 2–7 days to about 1 % acidity; sour starch ferments 20–70 days to about 5 % and is sun-dried, which gives it its oven expansion.
1978 Brazilian acidity limit
Titratable acidity of 5.00 ml NaOH N/100 g at most; 52 % of 52 commercial samples were over it with no loss of quality
1978 Brazilian moisture limit
14 % at most; 58 % of samples were over it
Organic acids in commercial sour starch
Lactic 0.42 g/100 g, butyric 0.06 g/100 g, acetic 0.02 g/100 g; water activity 0.57 on average
Oven expansion
3.0–8.6 ml/g across 13 Colombian genotypes after 30 days of fermentation and sun drying
Polvilho doceSweet starch, fermented 2–7 days; does not expand the same way.
Almidón agrio (Colombia)Colombian sour starch; fermented 30 days and sun-dried in the Alvarado study of 13 genotypes.
Safety
Bacillus cereus was found at low numbers in factory sour starch. Commercial samples averaged a water activity of 0.57, too dry for most microbes to grow in storage.
Hazards the sources name: Bacillus cereus, found at low numbers in product from both factories studied.