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Starters and koji from Benin

Yanyanku (Hibiscus-seed fermentation starter for sonru)yanyanku

Yanyanku is a Beninese additive made from roselle (bissap) seeds. The seeds are boiled for an hour in water with shea-wood ash filtrate, fermented in covered baskets, pounded and sun-dried. It is added near the end of the second boil of néré seeds to soften and seed them for sonru.

Fully documentedStarters and koji38 h – 4.1 days

Recipe

Amounts from a sourceThe ash strength is the study's recipe; the seed amount per batch is not given.
Roselle (bissap) seedsSorted and washed[agbo2019]
Shea-wood ash, as a filtered ash solution500 g per litre of waterMixed into the cooking water[agbo2019]
Starterspontaneous

Total time across the recorded steps: 38 h – 4.1 days

Start
Drag the bar or press Play
shapes, not to countBacillusLactic acid bacteria95–98 °C

What changes: Roselle seeds boil in alkaline ash water

Step 1 of 4
Boil the seeds in ash water
Time
1 h
Reported
Temperature
95–98 °C
Reported
Salt
None given
Acidity
Not documented
Which microbes the steps name, step by step
Bacillus
Lactic acid bacteria

Output: cooked seeds, drained.

Flavour
Not documented
Texture
seeds soften
Colour
Not documented

Microbes at work in this step

cooking selects for spore-forming Bacillus, though some lactic bacteria survive

Method

Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source

At a glance

Yanyanku is a Beninese additive made from roselle (bissap) seeds. The seeds are boiled for an hour in water with shea-wood ash filtrate, fermented in covered baskets, pounded and sun-dried. It is added near the end of the second boil of néré seeds to soften and seed them for sonru. Made in 4 steps over 38 h – 4.1 days. The longest step, ferment in covered baskets wrapped in jute sacks, takes 36 h – 4 days (Agbobatinkpo P.B 2019) and runs at 26–28 °C (Agbobatinkpo P.B 2019). Bacillus spp. and Lactic acid bacteria do most of the fermenting.

Key numbers
Main step36 h – 4 daysMeasured[agbo2019]Ferment in covered baskets wrapped in jute sacks
Temperature26–28 °CMeasured[agbo2019]Ferment in covered baskets wrapped in jute sacks
AciditypH 6.8 → 8.4Measured[agbo2019]Ferment in covered baskets wrapped in jute sacks

What makes this one different

Yanyanku is not a condiment itself but a ferment added to another one. Its sister additive ikpiru is made by boiling roselle seeds with Boerhavia erecta instead of ash and fermenting them in calabashes.

Related ferments

Variants

  • Ash-soak flow chart (Zannou et al.)Seeds soaked in ash solution for 7 days, decanted, sun-dried and ground; the chart (Fig. 2, after Azokpota et al. 2006) shows no boiling or basket stage.
  • Potash variants (Azokpota et al. 2011)Variant 1 adds potash before cooking, ferments 72 h then 24 h and sun-dries; variant 3 adds potash before cooking and ferments 7 days. Variant 2 adds ash solution after cooking, ferments 72 h and pounds and sun-dries twice. Final pH was 9.95, 8.23 and 10.14.

Safety

Agbobatinkpo et al. (2013) found Bacillus cereus among 355 spore-forming isolates from yanyanku and ikpiru and checked them for toxin genes (emetic cesB in 9 % of the B. cereus isolates). The ferment is aerobic with the pH rising to 8.4 (Agbobatinkpo et al. 2019). Azokpota et al. (2006) counted Staphylococcus rising to about 10^6 cfu/g in the sonru ferment made with yanyanku before it fell.

Hazards the sources name: Bacillus cereus (8–39 % of isolates from yanyanku and ikpiru); 9 % of the isolates carried the emetic toxin gene cesB and 15–35 % carried the hblA, hblC and hblD diarrhoeal toxin genes; Staphylococcus in the sonru ferment made with yanyanku, about 10^6 cfu/g at 12–18 h falling to 5 x 10^4 at 48 h.

History and sources

Every number is labelled with how it is known. How this atlas is made

Azokpota et al. (2006) followed sonru and iru fermentations, which run 48 h with yanyanku or iku-iru added. Azokpota et al. (2011) found three ways of making yanyanku and say the product is made at household level and is now also made commercially as sonru output has grown; their abstract gives a shelf life of about 10 years. Agbobatinkpo et al. (2011) found producers using Hibiscus sabdariffa, Gossypium hirsutum or Adansonia digitata seeds for yanyanku; their 2013 study identified the Bacillus species in it.

  1. [agbo2013] Agbobatinkpo P.B., Thorsen L., Nielsen D.S., Azokpota P., Akissoe N., Hounhouigan J.D., Jakobsen M. 2013, Biodiversity of aerobic endospore-forming bacterial species occurring in Yanyanku and Ikpiru, fermented seeds of Hibiscus sabdariffa used to produce food condiments in Benin, International Journal of Food Microbiology 163(2–3):231–238 (abstract)paper
  2. [azokpota2006] Azokpota P., Hounhouigan D.J., Nago M.C. 2006, Microbiological and chemical changes during the fermentation of African locust bean (Parkia biglobosa) to produce afitin, iru and sonru, three traditional condiments produced in Benin, International Journal of Food Microbiology 107(3):304–309 (abstract)paper
  3. [azokpota2011] Azokpota P., Houndenoukon M.S.E., Hounhouigan J.D., Nago M.C., Jakobsen M. 2011, Evaluation of yanyanku processing, an additive used as starter culture to produce fermented food condiments in Benin, Journal of Food Processing and Preservation 35(6):813–821 (abstract)paper
  4. [agbo2011] Agbobatinkpo P.B., Azokpota P., Akissoe N., Kayodé P., Da Gbadji R., Hounhouigan J.D. 2011, Indigenous perception and characterization of Yanyanku and Ikpiru, two functional additives for the fermentation of African locust bean, Ecology of Food and Nutrition 50(2):101–114 (abstract)paper
  5. [agbo2019] Agbobatinkpo P.B., Azokpota P., Hounhouigan J.D. 2019, Dynamique de la fermentation des graines de bissap pour la production du yanyanku et du ikpiru, Annales des Sciences Agronomiques 23(2):171–182paper
  6. [zannou2018] Zannou O., Chabi I.B., Koca I. 2018, Some traditional fermented food from African locust beans (Parkia biglobosa), production and volatile compounds, GSJ 6(9) (review; Fig. 1 and 2)paper