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Quinoa flour and water are mixed one to one and left warm, and a little of each day's ripe dough is used to start the next. Lactic acid bacteria from the flour take over and sour it within a few days, and the stable sourdough is then added to wheat bread dough.
Fully documentedCereals and bread8 days – 10 days
Recipe
Amounts from a sourceProportions of the laboratory sourdough of Franco et al. 2020. No household recipe with fixed amounts was found; the 20 % dose for wheat bread is from Rizzello et al. 2016.
Quinoa flour1 part by weight
Desaponified and milled to wholegrain flour[franco2020]
backslop (spontaneous at first); selected strains in some studies
Total time across the recorded steps: 8 days – 10 days
Start
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What changes:Quinoa grains are desaponified and milled into wholegrain flour
Step 1 of 4
Remove the saponins and mill the grain to wholegrain flour
Time
Not documented
Temperature
Not documented
Salt
None given
Acidity
Not documented
Which microbes the steps name, step by step
Lactic acid bacteria
In the laboratory study the milled flours were kept in sterile containers at 4 °C until use. Output: wholegrain quinoa flour.
Flavour
Not documented
Texture
Not documented
Colour
Not documented
Microbes at work in this step
Not documented for this step.
Method
Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source
At a glance
Quinoa flour and water are mixed one to one and left warm, and a little of each day's ripe dough is used to start the next. Lactic acid bacteria from the flour take over and sour it within a few days, and the stable sourdough is then added to wheat bread dough. Made in 4 steps over 8 days – 10 days. The longest step, ferment warm and back-slop every day, takes 8–10 days (Franco W 2020) and runs at 30 °C (Franco W 2020). Pediococcus pentosaceus and Lactobacillus, Leuconostoc (L. citreum), Weissella (W. cibaria), Lactococcus and Enterococcus in spontaneous quinoa sourdough do most of the fermenting.
Quinoa has no gluten. In the spontaneous doughs of Franco et al. 2020, lactobacilli, pediococci and enterococci were the most common isolates; among the bacteria, only the pediococci, leuconostocs and weissellas made exopolysaccharides (thickening sugars) from starch, and P. pentosaceus gave the highest yield.
Amaranth sourdoughAmaranth flour back-slopped daily at 30 °C for 10 days; L. plantarum, L. sakei and P. pentosaceus were selected as starter candidates.
Selected-starter quinoa sourdoughStarted with L. plantarum T6B10 and F. rossiae T0A16 isolated from quinoa instead of waiting for spontaneous strains.
Safety
Back-slopped quinoa doughs reached pH about 4 within a few days in the laboratory (Franco et al. 2020). The bread is baked before eating.