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Starters and koji from Okinawa

Black koji (kuro-koji)黒麹

Black koji is steamed rice grown with Aspergillus luchuensis, a black-spored koji mould that makes a lot of citric acid. In the Okinawan trial conditions it is grown for 42 hours, at 40 °C first and cooler at the end, so the acid builds up. Awamori is brewed from black rice koji alone, and the acid keeps spoilage bacteria out of the warm mash.

Fully documentedStarters and koji42 hFamily: Rice koji for sake (yellow koji, kome-koji)

Recipe

No fixed amounts recordedNo amounts are given; the GI rules fix the mould and the all-koji method, not a batch size.
Rice, steamedThai rice is used for the rice koji in the Okinawan trial-brew list[oki2021]
Black koji seed (Aspergillus luchuensis)Awamori under the Ryukyu designation must use only rice koji grown with A. luchuensis[nta-ryukyu][oki2021]
Starterdefined-culture: black koji spores (Aspergillus luchuensis; A. awamori in older literature)

Total time across the recorded steps: 42 h

Start
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What changes: Rice is steamed and cooled

Step 1 of 5
Steam the rice
Time
Not documented
Temperature
Not documented
Salt
None given
Acidity
Not documented
Which microbes the steps name, step by step
Aspergillus luchuensis
Moulds

Output: steamed rice, cooled for seeding.

Flavour
Not documented
Texture
Not documented
Colour
Not documented

Microbes at work in this step

Not documented for this step.

Method

Measured measured in a studySet fixed by a specification or tested recipeReported one source reports itTypical a general value, not sourced for this fermentUnconfirmed not yet checked against a source

At a glance

Black koji is steamed rice grown with Aspergillus luchuensis, a black-spored koji mould that makes a lot of citric acid. In the Okinawan trial conditions it is grown for 42 hours, at 40 °C first and cooler at the end, so the acid builds up. Awamori is brewed from black rice koji alone, and the acid keeps spoilage bacteria out of the warm mash. Made in 5 steps over 42 h. The longest step, mix and cool so citric acid builds up, takes 22 h (Okinawa Prefecture 2021) and runs at 25–35 °C (Hayashi K 2021). Aspergillus luchuensis does most of the fermenting.

Key numbers
Main step22 hReported[oki2021]Mix and cool so citric acid builds up
Temperature25–35 °CReported[hayashi2021][oki2021]Mix and cool so citric acid builds up

What makes this one different

Black koji makes much more citric acid than the yellow koji of sake, so the mash stays sour and safe in Okinawa's hot, humid climate. Its black colour is the main thing white koji, an albino mutant picked out of it, lacks.

Related ferments

Variants

  • White kojiAn albino mutant of the black koji mould that keeps its enzymes and citric acid; see its own entry.

Made the black koji way

Safety

The citric acid the black koji makes keeps the mash pH low and holds back microbes other than the yeast. Black koji belongs to Aspergillus section Nigri, which also holds A. niger; some A. niger strains make ochratoxin or fumonisin, but Hayashi et al. state that the three koji fungi used for shochu and awamori cannot make mycotoxins.

History and sources

Every number is labelled with how it is known. How this atlas is made

The scientific name Aspergillus luchuensis comes from Ryukyu, and the mould is named a "national fungus" (kokkin). Kagoshima shochu makers brought black koji over from Okinawa around 1910. Molecular work showed the old name A. awamori covered several species, and the awamori mould was renamed A. luchuensis.

  1. [nta-ryukyu] National Tax Agency of Japan, geographical indication 琉球 (Ryukyu) production standard (地理的表示「琉球」生産基準), amended 2020-09-14spec
  2. [oki2021] Okinawa Prefecture, 2021 trial-brew library list (2021試醸酒ライブラリリスト), status 2021-03-31, with the base koji-making conditionsgov
  3. [hayashi2021] Hayashi K., Kajiwara Y., Futagami T., Goto M., Takashita H. 2021, Making traditional Japanese distilled liquor, shochu and awamori, and the contribution of white and black koji fungi, Journal of Fungi 7(7):517paper